Characterization of two glutamate dehydrogenases from the symbiotic microalga Symbiodinium microadriaticum isolated from the coral Acropora formosa

In view of their possible involvement in ammonium assimilation in the coral/algal symbiosis, we have purified two distinct glutamate dehydrogenase isoenzymes from the symbiotic dinoflagellate Symbiodinium microadriaticum (Freudenthal) extracted from the staghorn coral Acropora formosa collected from Magnetic Island, North Queensland, Australia, in 1986–1987. An NADPH-specific glutamate dehydrogenase (GDH) displayed biphasic kinetics with respect to ammonium as the variable substrate; at low substrate concentrations the apparent K _m was below 1 mM, whereas at high substrate concentrations the corresponding value was approximately 200 mM. The NADPH-GDH displayed extremely low activity in the direction of glutamate oxidation; together with the kinetic data this suggests a probable role in ammonium assimilation. A second (NADH-specific) GDH was found to have both amination and deamination activities, and presumably functions in vivo in glutamate oxidation. Kinetic constants are reported for both GDH isoenzymes.     

Farm factors associated with reducing Cryptosporidium loading in storm runoff from dairies

A systems approach was used to evaluate environmental loading of Cryptosporidium oocysts on five coastal dairies in California. One aspect of the study was to determine Cryptosporidium oocyst concentrations and loads for 350 storm runoff samples from dairy high use areas collected over two storm seasons. Selected farm factors and beneficial management practices (BMPs) associated with reducing the Cryptosporidium load in storm runoff were assessed. Using immunomagnetic separation (IMS) with direct fluorescent antibody (DFA) analysis, Cryptosporidium oocysts were detected on four of the five farms and in 21% of storm runoff samples overall. Oocysts were detected in 59% of runoff samples collected near cattle less than 2 mo old, while 10% of runoff samples collected near cattle over 6 mo old were positive. Factors associated with environmental loading of Cryptosporidium oocysts included cattle age class, 24 h precipitation, and cumulative seasonal precipitation, but not percent slope, lot acreage, cattle stocking number, or cattle density. Vegetated buffer strips and straw mulch application significantly reduced the protozoal concentrations and loads in storm runoff, while cattle exclusion and removal of manure did not. The study findings suggest that BMPs such as vegetated buffer strips and straw mulch application, especially when placed near calf areas, will reduce environmental loading of fecal protozoa and improve stormwater quality. These findings are assisting working dairies in their efforts to improve farm and ecosystem health along the California coast.     

Comparative study of 137Cs partitioning between solid and liquid phases in Lakes Constance, Lugano and Vorsee.

The methodology for estimating radiocaesium distribution between solid and liquid phases in lakes is applied for three prealpine lakes: Lake Constance (Germany), Lake Lugano (Switzerland) and Lake Vorsee (Germany). It is based on use of the exchangeable distribution coefficient and application of the exchangeable radiocaesium interception potential (RIPex). The methodology was tested against experimental data. Good agreement was found between estimated and measured 137Cs concentrations in Lake Constance and Lake Lugano, whereas for Lake Vorsee a discrepancy was found. Bottom sediments in Lake Vorsee are composed mainly of organic material and probably cannot be described in terms of the specific sorption characteristics attributed to illitic clay minerals.     

The prebiotic synthesis of pyrimidines in frozen solution

Most prebiotic syntheses depend on the reaction of concentrated precursor compounds to produce bio-organic molecules. It is now believed that the early Earth’s atmosphere was not reducing enough to have permitted copious synthesis of precursor molecules. Freezing allows reaction to occur even from dilute solution. This reaction has been demonstrated for the purines but not for the pyrimidines. It is shown here that dilute solutions of simple prebiotic molecules produce the biological pyrimidines cytosine and uracil upon freezing. Cold environments may have allowed synthesis of all of the RNA bases even from low organic yielding atmospheres, such as those of the early Earth, Mars, Titan and Europa.     

The risk assessment-policy gap: an example from the UK contaminated land regime

Risk-based regulation assumes that risk assessment can evaluate risks against policy objectives. However, policy goals are often ambiguous and require risk assessors to interpret them for use in risk assessment. This risk assessment-policy gap stems partly from normative and imprecise policy language but is rooted more fundamentally in society's uncertain expectations for the environment. Until this uncertainty is resolved, the democratic and regulatory effectiveness of risk regulation will be undermined by ad hoc policy decisions abdicated to risk assessors.     

Hybridization within the species complex of the scleractinan coral Montastraea annularis

The morphologically variable reef coral previously known as Montastraea annularis (Ellis and Solander, 1786) has recently been separated into three species based on differences in morphology, behavior, allele frequencies and some life-history traits of Panamanian specimens. To further investigate the proposed reclassification and its conformity to the biological species concept we conducted reciprocal intra- and inter- specific fertilization experiments with gametes from each of the three species on Florida reefs. With one exception, self-fertilization rates were very low or zero. Within-species crosses resulted in production of planulae, as did all inter-species (hybrid) crosses, but there was much variation in fertilization success within each type of cross. In an experiment with separated gametes, hybrid crosses between M. annularis and M. franksi produced more larvae than within-species crosses for each species. Hybridization crosses between M. faveolata and the other two species produced fewer larvae than did within- M. faveolata crosses in the experiment with separated gametes, but many larvae resulted when the hybridizations were performed by mixing entire gamete bundles. Additional observations showed that M. franksi had 20% larger eggs and fewer eggs per gamete bundle than did the other two species and that it consistently spawned 1 to 1.5 h before the others, a potential temporal barrier to hybridization. These results indicate that there is no inherent pre-zygotic barrier to cross-fertilization among the three morphological species, although post-zygotic survival and fertility remain to be determined. The adherence of the proposed reclassification to the biological species concept requires further examination. Received: 16 April 1997 / Accepted: 17 June 1997     

Distribution and ecology of leptocephali of the congrid eel, Ariosoma scheelei, around Sulawesi Island, Indonesia

A survey for leptocephali around Sulawesi Island in the central Indonesian Seas during May 2001 found that the leptocephali of the congrid eel, Ariosoma scheelei, were present in all seven areas that were sampled. A total of 551 leptocephali (22–166 mm TL) were collected, and A. scheelei was by far the most abundant species of leptocephali collected during the survey. The wide range of sizes in most areas indicated that spawning had occurred during a period of several months in many different areas, although the exact spawning locations were not determined. The larger size classes were more abundant in all areas except in Tomini Bay on the northeast side of Sulawesi Island. The highest catch rates were observed at the eastern edge of the Java Sea and to the north in the Celebes Sea near Makassar Strait. Premetamorphic leptocephali were also collected in surface samples at 11 stations (N=62), but metamorphosing leptocephali (N=86) were only caught in IKMT tows that fished from the surface to about 200 m. Metamorphosing leptocephali were collected primarily at two stations in the Java Sea and Makassar Strait where a surface layer of lower-salinity water was detected. Their total lengths (105.3–153.3 mm) and the largest premetamorphic individuals suggested that this species can reach maximum sizes of about 165 mm before beginning to metamorphose. It is hypothesized that this species may be abundant in the Indonesian Seas region and that it has ecological traits such as large size at recruitment and a small size at reproduction that have made it successful in many regions of the Indo-Pacific.